ROS imaging shows that Hvegt1 root basal meristem and elongation zone tissues have reduced levels. Transcript profiling revealed Hvegt1 roots deregulate reactive oxygen species (ROS) homeostasis and cell wall-loosening enzymes and cofactors. Gravitropic assays indicated that Hvegt1 roots bend more rapidly than wild-type. Haplotype analysis found that natural allelic variation at the barley EGT1 locus impacts root angle. EGT1 encodes an F-box and Tubby domain-containing protein that is highly conserved across plant species. Mutations in barley and wheat EGT1 genes confer a striking root phenotype, where every root class adopts a steeper growth angle. Here we report a root angle regulatory gene termed ENHANCED GRAVITROPISM1 ( EGT1) that encodes a putative AGO component, whose loss-of-function enhances root gravitropism. Root angle is determined by competing gravitropic versus antigravitropic offset (AGO) mechanisms. Root angle in crops represents a key trait for efficient capture of soil resources. Bennett, Rahul Bhosale, and Silvio Salvi -31 Authors Info & Affiliations Nagel, Laura De Gara, James Simmonds, Cristobal Uauy, Roberto Tuberosa, Jonathan P. Tucker, Axel Himmelbach, Nils Stein, Martin Mascher, Kerstin A. Mooney, Frank Hochholdinger, Matthew R. Kirschner, Ranjan Swarup, Alberto Tassinari, Bipin K. Borkar, Marco Lombardi, Cristian Forestan, Sara G. Riccardo Fusi, Serena Rosignoli, Haoyu Lou, +31, Giuseppe Sangiorgi, Riccardo Bovina, Jacob K.

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